Extended Data Fig. 4: Generation of the rice mutants of IRX10 homologues.
From: Xylan-based nanocompartments orchestrate plant vessel wall patterning
a, Diagram of IRX10 and its homologous genes. The mutant alleles are indicated by short bars. b, The mature plants of the indicated genotypes. c, Microscopy images of the large vein in wild type and the indicated mutants. The boxed parts were magnified in Fig. 2b. d, Transmission electronic microscopy images of vessel walls in the indicated genotypes. Asterisks indicate the vessel walls. e-g, Boxplot of diameter (e), length (f), and wall thickness (g) of vessels in the indicated genotypes. Box boundaries represent the 25th and 75th percentile, centre line represents the median, × indicates the mean, and whiskers represent the 25th percentile − 1.5 * the interquartile range and the 75th percentile + 1.5 * the interquartile range. P < 0.0001 by one-way ANOVA. n = 50 in (e) and 21~47 in (f) of vessels from three individual indicated plants. In (g), n = 100 measurements in at least four vessels from three individual indicated plants. Letters a–e indicate statistical significance according to Tukey’s multiple range test. P < 0.05. Tm1, Tm2, Tm3, and Qm indicate triple and quadruple mutants defined in Extended Data Fig. 3b. Bars = 12 cm in (b), 20 μm in (c) and 1 μm in (d).
