Extended Data Fig. 7: Enzyme activity assay of rice IRX10.
From: Xylan-based nanocompartments orchestrate plant vessel wall patterning
a, The mutating amino acids examined in this study were indicated in the predicted 3D structure of rice IRX10. b, Recombinant proteins of rice IRX10 and the variants (Upper panel). Western blotting of IRX10 and the variants using anti-His antibodies (Low panel). 10D represents D311N/D312N. 10R indicates R295K. CBB, Coomassie brilliant blue staining. c,d, Electrophoretograms of the reaction products generated by IRX10 and its variants using UDP-xylose (c) and xylopentaose plus UDP-xylose (d) as the substrates, respectively. Xyl1‒Xyl8 indicate standard markers of xylo-oligosaccharides. Asterisks indicate unspecific bands. e, Activity analysis of recombinant IRX10 and the variants. ++ indicates more activated than +; − indicates undetectable activity. The experiments in (b-d) were repeated independently for at least three times.
