Extended Data Fig. 1: Depositing xylan in the developing rice internodes.
From: Xylan-based nanocompartments orchestrate plant vessel wall patterning
a, Diagram of a developing wild type (Nipponbare, NP) internode (left), a large vein (centre), and a macerated metaxylem vessel (right). S1‒S9 indicate the sequential segments of internode from the base to the tip. SCW, Secondary cell wall. b, Xylan abundance in each segment, analysing the monosaccharide content. Error bars represent the mean ± s.d. n = 5 biological replicates. c, Representative electrophoretogram of the released xylo-oligosaccharides. d, Xylo-oligosaccharides released by xylanase M6 digestion. Error bars represent the mean ± s.d. n = 3 biological replicates. e, Morphology of vessel pits in the NP developing internodes. The vessel walls were stained with S4B. f, Boxplot of pit size of vessel walls shown in (e). Box boundaries represent the 25th and 75th percentile, centre line represents the median, × indicates the mean, and whiskers represent the 25th percentile − 1.5 * the interquartile range and the 75th percentile + 1.5 * the interquartile range. P < 0.0001 by one-way ANOVA. n = 200 vessel pits from three individual plants. Letters a–d indicate statistical significance according to Tukey’s multiple range test. P < 0.05. Bars = 1 cm in (a) and 2 μm in (e).
