Fig. 4: Co-transcriptional and post-transcriptional processing co-exist for most pri-miRNAs.
From: R-loops at microRNA encoding loci promote co-transcriptional processing of pri-miRNAs in plants
a, Schematic representation of the positions used to quantify co-transcriptional versus post-transcriptional processing ratios. In all cases, total number of reads ending in the nt marked as 'a' (green lines) were expressed relative to reads expanding this site (blue lines). b, Co-transcriptional processing ratios corresponding to all analysed miRNAs in two independent plaNET-seq experiments and split by processing mechanism. c, Boxplot representation of the co-transcriptional processing ratios. Red line marks ratio = 1 where co- and post-transcriptional processing are equally frequent. ExpA and ExpB show two independent experiments, respectively, of n = 2 biologically independent samples each. Dots represent single data points, whiskers denote the minimum/maximum values (no further than 1.5× interquartile range (IQR) from the hinge), the box defines the IQR, the centre represents the median and box bounds represent the lower and upper quartiles. d, Fraction of pri-miRNAs preferentially processed co-transcriptionally, post-transcriptionally or with equal preference. Two independent experiments in control conditions.
