Extended Data Fig. 4: CP-DIPSHIFT experiment.

CP-DIPSHIFT curves of WT (black) and gosamt1 gosamt2 (red) cell walls measured at 293 K. Best-fit 13C-1H dipolar coupling values (scaled by FSLG) and SCH order parameters are given in each panel. The experiment was conducted with C-H dipolar doubling, a CP contact time of 500 μs under 7.8 kHz MAS. The 101 ppm, 99.0 ppm, and 79.8 ppm peaks of pectin backbones are more rigid in the mutant than in the WT cell wall.