Extended Data Fig. 2: 13C3-Pyruvate and malate feeding to isolated mitochondria of Col-0, mpc1 and mpc1/gMPC1.
From: Metabolic evidence for distinct pyruvate pools inside plant mitochondria
Time courses of metabolite concentrations in the extra-mitochondrial space of isolated mitochondria incubated with 500 µM 13C3-pyruvate and 500 µM malate via MPC pathway (a) and via NAD-ME pathway (b). All experiments were conducted in the presence of ADP at pH 6.4 to initiate substrate uptake and consumption by both pathways. Metabolic reaction was stopped by centrifugation through a single silicon oil layer in which the mitochondrial pellet was separated from the extra-mitochondrial medium. Unused substrate and exported products in the extra-mitochondrial medium were quantified using LC-SRM-MS. Each data point represents averaged value from three biological replicates with error bars indicating standard error (n = 3). Significant differences between mpc1, Col-0 and mpc1/gMPC1 are denoted by asterisks based on two-sided Student’s t-tests (*, p < 0.05, See Source data Extended data Fig. 2 for exact p-values).
