Extended Data Fig. 6: SILIA IP-MS PRM analysis of flg22-induced BSU1 phosphorylation.

(a) Chromatograms of fragment ions from the replicate SILIA IP-MS PRM experiment of Fig. 3c using reciprocal labeling. (b) The ratio of phosphorylated and non-phosphorylated MDSDNVWTPVPAVAPpSPR peptides. The values of peak area were used for quantification. The ratios of S251 phosphorylation between flg22-treated (+flg22) and mock-treated (-flg22) samples are shown.