Extended Data Fig. 8: Relative band intensity of Fig. 5a, c.
(a) Relative band intensities of immunoblot images of flg22-treated samples using anti-phospho-p44/42 MAPK antibody (anti-p-MPK) in Fig. 5a were measured by ImageJ. The band intensity of each bsu-i sample was set to 1. The graph shows the average band intensity ± SEM from three repeat experiments. One-tail t-tests show that the band intensities of BSU1;i-1 and BSU1;i-2 are significantly stronger than that of S251A;i (p = 0.0268 and p = 0.0157). (b) Band intensities of immunoblot images, represented by Fig. 5c, were measured by ImageJ. The band intensity of GST-BIN2 was divided by that of MBP-BSU1, and then the ratio between +GST-CDG1 and -GST-CDG1 is presented to show the effect of CDG1 on BSU1-BIN2 binding. The graph shows the average binding activity ± SEM from three repeat experiments. One-tail t-test shows that the binding activity of S251A is significantly higher than that of S764A. (p = 0.041983).
