Extended Data Fig. 4: The flg22- and elf18-induced MPK3/6 phosphorylation in the BSU1 family mutants.
(a) Flg22-induced MPK3/6 phosphorylation in wild-type (WT) and bsl2;bsl3. Ten-day-old seedlings were treated with 100 nM flg22 for 10 min. Immunoblot was performed using anti-phospho-p44/42 MAPK and anti-BSL2/3 antibodies. Ponceau S staining shows equal protein loading. (b) Elf18-induced MPK3/6 phosphorylation in bsu-i. Twelve-day-old seedlings grown on 20 µM estradiol containing media were treated with 1 µM elf18 for 10 min. Immunoblot was performed using anti-phospho-p44/42 MAPK and anti-BSL2/3 antibodies. Ponceau S staining shows equal protein loading.
