Extended Data Fig. 2: Principal Component Analysis of the CO2 concentrating mechanism proxies analysed.
From: Correlative adaptation between Rubisco and CO2-concentrating mechanisms in seagrasses
Variables included are: isotopic discrimination of 13C of leaf biomass (δ13C), pH compensation point determined by pH-drift assay (pH*), in vivo photosynthetic semi-saturation constant for CO2 (Km CO2) and for dissolved inorganic carbon (Km DIC), percentage of the net photosynthetic rate inhibition after addition of acetazolamide (AZ), ethoxyzolamide (EZ), TRIS-buffer (TRIS) and 4,40-diisothiocyanatostilbene-2,20-disulfonate (DIDS), and the percentage of the net photosynthetic rate variation at pH 9-9.5 (An rate at high pH) and at pH 7-7.5 (An rate at low pH) relative to water environmental pH (pH ~8).
