Extended Data Fig. 9: Mutations in XRN4 can rescue the heat hypersensitivity of alba456-2.
From: ALBA proteins confer thermotolerance through stabilizing HSF messenger RNAs in cytoplasmic granules
a, Schematic presentation of the Arabidopsis XRN4 gene. Untranslated and coding regions are depicted as thick gray and blue boxes, respectively. Introns are depicted as thin black boxes. Triangles indicate the CRISPR/Cas9 target sites and T-DNA insertion position in XRN4. The XRN4 gene is drawn to scale. The sgRNA target sequences are highlighted in yellow. The PAM sites are underlined. b, Photographs of Col-0, alba456-2, xrn4-5 and alba456-2 xrn4-9 seedlings under control (22 °C) and prolonged heat treatment (37 °C for 5 d). Scale bars = 2 cm. c, Quantification of the data shown in (b); the percentage of seedlings in different phenotypic classes was determined for different genotypes and different conditions. Data are presented as the mean ± SD of three biological replicates. Significant differences between two groups are marked with different letters as determined by two-sided Fisher’s exact test (P < 0.01). d, Decay profiles of HSF mRNAs under heat stress conditions (37 °C for 12 h). ALBA5 non-target AT2G07689 was used as the negative control. The mRNA decay profile of each genotype is presented as the relative mRNA abundance after transcription inhibition by cordycepin. Results from one representative replicate of two independent biological replicates are shown (each in three technical replicates). Data represent mean ± SD. See Source Data for P values.
