Extended Data Fig. 1: Interactions between ALBA4/ALBA5/ALBA6 and DCP5.
From: ALBA proteins confer thermotolerance through stabilizing HSF messenger RNAs in cytoplasmic granules
a, Yeast two-hybrid analysis of the interactions between ALBA proteins and PB and SG components. Combinations of constructs were transformed into yeast strain AH109 and assayed on medium lacking Leu and Trp (SD-TL), medium lacking Leu, Trp, and His (SD-TLH), and medium lacking Leu, Trp, His and Ade (SD-TLHA). AD, activating domain; BD, binding domain; Vec, empty vector. Data are representative of three independent experiments. b, Co-IP results showing the interactions between ALBA proteins and DCP5 in vivo. Transgenic plants expressing ALBA4-Myc or ALBA5-Myc and F1 offspring seedlings (ProALBA4:ALBA4-Myc × ProDCP5:DCP5-GFP and Pro35S:ALBA5-Myc × ProDCP5:DCP5-GFP) were used for Co-IP. Data are representative of two independent experiments with similar results.
