Extended Data Fig. 2: ALBA4, ALBA5 and ALBA6 reversibly localize to cytoplasmic mRNP granules under heat stress.

a, b, Fluorescence images showing colocalization of ALBA6 with ALBA4 (a) and ALBA5 (b). The root tissue of 5-day-old F1 seedlings co-expressing the indicated transgenes under control (22 °C) and heat stress (39 °C for 50 min) conditions was used for the experiments. Scale bars = 10 μm. Images are representative of three independent replicates, and at least five roots were monitored for each condition and replicate. c, Fluorescence images showing localization of ALBA4, ALBA5, ALBA6 and Rbp47b during recovery from heat stress. The transgenic plants were pretreated at 39 °C for 50 min, after which images of root tip cells were taken at 22 °C at the indicated time points. Scale bars = 10 μm. d, Quantification of ALBA4, ALBA5, ALBA6 and Rbp47b foci in (c). Data represent the mean ± SD. For ALBA4-GFP foci, n = 17, 11, 14, 14 and 10, for 10, 30, 50, 70 and 90 min, respectively; for ALBA5-GFP foci, n = 13, 18, 12, 17 and 8, for 10, 30, 50, 70 and 90 min, respectively; for ALBA6-GFP foci, n = 18, 23, 29, 26 and 24, for 10, 30, 50, 70 and 90 min, respectively; for Rbp47b, n = 30, 23, 25, 18 and 15, for 10, 30, 50, 70 and 90 min, respectively.