Extended Data Fig. 1: Constructs of SOQ1 used in the present study. | Nature Plants

Extended Data Fig. 1: Constructs of SOQ1 used in the present study.

From: Structure of Arabidopsis SOQ1 lumenal region unveils C-terminal domain essential for negative regulation of photoprotective qH

Extended Data Fig. 1

(A) Domain structure of SOQ1. SOQ1 contains chloroplast transit peptide (cTP; green), HAD domain (yellow), transmembrane region (TM; blue), Trx-like domain (cyan) containing the CCINC motif, NHL domain (bright orange) and CTD (hot pink). Numbers indicate amino acid positions. (B) Constructs of SOQ1 truncations used for crystallization, crosslinking and cryo-EM. The region built in each crystal structure is shown below its corresponding construct (framed in one rectangular box). Mutated residues are indicated. (C) Construct of SOQ1 truncations used for plant transformant lines. (D) Constructs of SOQ1 truncations for microscale thermophoresis (MST) experiment. (E) Constructs of SOQ1 truncations used as controls for heterogeneous degradation.

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