Extended Data Fig. 5: Module 1 also groups genes with an extended or partial ring expression pattern.
From: A root phloem pole cell atlas reveals common transcriptional states in protophloem-adjacent cells
a) New genes with an expression pattern validating the module eigengene analysis. All the genes presented in this panel are expressed forming a ring pattern (At3g1633802 or extended ring (At4g27435, note the expression in protoxylem, and PER30, note the expression in procambium) at the time of PSE enucleation. They are all grouped in module 1, except At4g27435, which belongs to module 4. UMAPs show the particular cluster-weighted normalised expression of each gene in the phloem pole cell atlas and microscopy pictures are representative images of the transcriptional reporter lines where the gene promoter is fused to VENUSer. Scale bar in the longitudinal sections is 25 µm while it is 10 µm in the cross sections. White arrowheads point to PSE cells as a reference point. Each gene has also been plotted in PPP (green), CC (orange) and PSE (purple) trajectories, showing average expression values in the Y-axis and pseudotime in the X-axis. The numbers in each panel indicate samples with similar results, of the total independent biological samples observed.
