Extended Data Fig. 6: Phenotypic analyses of duo1 mutants in the field in Zhaoxian, Hebei Province, China in 2019/20 at the sowing density of 180 grains/m2.
From: Improving bread wheat yield through modulating an unselected AP2/ERF gene
a, CRISPR/Cas9-based mutations in the DUO-B1 gene. The positions and sequences of target sites for CRISPR/Cas9 gene editing are shown. The symbols ‘-’ indicated the nucleotide deletion with the base numbers of deletion shown behind. nt, nucleotide. b, Comparisons of the grain width and grain length between Fielder and two independent mutant lines, duo-B1_m1 and duo-B1_m2. Bars, 1 cm. c to e, Comparisons of the spike fertility index c, harvest index d, 1000-grain weight e, between Fielder and two independent mutant lines, duo-B1_m1 and duo-B1_m2. Data are shown as mean ± s.d.; dots show data distribution (n = 20 biologically independent samples, P values calculated using two-tailed t-test).
