Extended Data Fig. 3: Subcellular and tissue localization of the transport and biosynthetic genes.
From: Root-secreted bitter triterpene modulates the rhizosphere microbiota to improve plant fitness
a, Subcellular localization of the two MATEs in cucumber protoplasts. CmMATE1-GFP or ClMATE1-GFP fluorescence is co-localized with a known Arabidopsis plasma membrane marker PIP2A-RFP fluorescence. From left to right: GFP signal (green), RFP signal (red), Differential Interference Contrast (DIC), and overlay of three signals (green, red and DIC) of the same protoplast. b and c, In situ localization of CmBi (b) or ClBi (c) transcripts in the outer region of young root tips in melon and watermelon, respectively. Both transverse (above the broken line) and longitudinal (below the broken line) sections of the root tip are presented. d, Subcellular localization of CmMATE1-GFP or ClMATE1-GFP fusion protein in yeast. All MATE-GFP fusion proteins were localized to the yeast tonoplast. From left to right: GFP fluorescence (green), FM4-64 staining of the vacuolar membrane (red), Differential Interference Contrast (DIC), merged images with GFP, FM4-64 and DIC from the same sample. For a to d, Three independent experiments were repeated with similar results.
