Extended Data Fig. 6: flg22 triggers ROS production in protoplasts in a manner dependent on FLS2 and RBOHD.

Protoplasts were prepared from the indicated plants, treated with 1 µM flg22, and ROS was measured immediately and expressed as relative luminescence units. Each data point represents mean ± sd, n ≥ 8 technical replicates (200 μl of protoplasts as one sample). This experiment was repeated three times with similar results.