Extended Data Fig. 2: PRXIIs and GPXs are required for stomatal immunity. Gene editing of peroxidases.
a, Mutations of GPX2, GPX5, and GPX6 in the gpx2 gpx5 gpx6 (gpx2-2 gpx5-1 gpx6-3) triple mutant generated by CRISPR-Cas9. b, Mutations in the prxIIB-1 single mutant and prxIIB prxIIC prxIID (prxIIB-1 prxIIC-1 prxIID-1) triple mutant generated by CRISPR-Cas9. Nucleotides in red indicate single base insertion in the mutants. c, The prxIIB single mutant and prxIIB prxIIC prxIID triple mutant are impaired in the restriction of bacterial entry. This experiment was repeated three times with similar results. d, Accumulation of PRXIIB in prxIIB and prxIIB prxIIC prxIID plants. PRXIIB protein was detected by immunoblotting with anti-PRXIIB antibodies. This experiment was repeated three times with similar results. e, PRXIIB restores stomatal immunity to the prxIIB prxIIC prxIID triple mutant. f, The gpx2 gpx5 gpx6 mutant is mildly affected in the restriction of bacterial entry. For experiments in c, e, and f, leaf discs of indicated plants were soaked with Pst DC3000 cor − for 1.5 h, and then the bacterial number was determined. Different letters indicate significant difference at P < 0.05. Data are mean ± sd (n = 12, one-way ANOVA, Tukey post-test). Each experiment was repeated at least three times with similar results.
