Extended Data Fig. 1: Expression of MUCI70Δ77 in HEK293 cells.
From: Polymerization of the backbone of the pectic polysaccharide rhamnogalacturonan I
MUCI70Δ77 was expressed in a total of two small-scale (20 mL) and one large-scale (250 mL) cultures. Total protein is the measure of fluorescence of total GFP fluorescence from cells + culture medium. Secreted protein is the measure of fluorescence of cell-free medium. All samples were taken from a 100 µL aliquot from the cell culture after 6 days. MUCI70Δ77 was expressed with 93% secretion efficiency, defined as the proportion of secreted protein to the total protein fluorescence. Error bars represent the standard deviation from three biological replicates.
