Extended Data Fig. 2: Digest of RG-I mucilage and purification of RG-I acceptor oligosaccharides. | Nature Plants

Extended Data Fig. 2: Digest of RG-I mucilage and purification of RG-I acceptor oligosaccharides.

From: Polymerization of the backbone of the pectic polysaccharide rhamnogalacturonan I

Extended Data Fig. 2

a, Arabidopsis mucilage was digested for the indicated times with RG-I hydrolase and by acid hydrolysis. Digests were carried out using 10 mg of mucilage and 0.1 µg RG-I hydrolase from Aspergillus aculeatus at 40 °C or 0.1 M HCl at 80 °C for the indicated times. b, RG-I oligosaccharides from digested mucilage were injected into a CarboPac PA-1 semi-preparative (22×250 mm) column following labeling with 2AB. Fractions were collected as individual peaks containing RG-I oligosaccharides of the indicated degree of polymerization (indicated above peak). Peaks were eluted in a gradient ranging from 50-1000 mM ammonium formate indicated by the green line.

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