Extended Data Fig. 10: The predicted secondary structures of YABBY1 B-region in cucurbits.
From: Architecture design of cucurbit crops for enhanced productivity by a natural allele
The secondary structures of B-region of CmoYABBY1 (a), CsYABBY1 (b) and ClYABBY1 (c) were predicted by using UNAFold software. (d) Two mutant constructs were used to detect the effect of predicted stem-loop structure on protein translation by determining the LUC/REN activity and mRNA levels. The modified-1 mutant construct was designed to enhance the stem-loop, and the modified-2 mutant construct was designed to destroy the stem-loop. A wild type (WT) construct and those carrying modified-1 or modified-2 constructs were expressed in Cucurbita moschata cotyledons. The mean LUC/REN activity and mRNA levels conferred by each mutant construct were normalized to those of WT control (n = 8 biological replicates). Data are presented as mean ± SD. Different letters indicate significant differences (P < 0.05, one-way ANOVA and Tukey’s test). (e) The secondary structure of mutant B-region (modified-1) of CmoYABBY1 was predicted by UNAFold software.
