Extended Data Fig. 9: Visualization of HNO in Arabidopsis - fluorescence versus bright-field images.
From: Discovery of endogenous nitroxyl as a new redox player in Arabidopsis thaliana
a-c, Representative confocal laser scanning fluorescence microscopy (top panel) and bright-field (bottom panel) images of a cross-section of Arabidopsis leaf or a cell suspension stained with 16 μM phosphine-based fluorescent probe (Ex: 488 nm, Em: 505–530 nm) in the absence (control) or presence of CM (1.5 mM), TXPTS (5 mM), or CM+ TXPTS, respectively. a, Arabidopsis WT leaves; b, noa1-2, nia1nia2, nia1nia2noa1-2 – NO deficient mutants and cell suspension; c, Arabidopsis WT leaves at 48 h of hypoxia stress. Red arrows indicate vascular tissues; scale bars, 100 μm; cell suspension, 40 μm. For confocal observation the leaf cross-sections were randomly selected from ~ 20 slices pooled from leaves of different plants of the same genotype. The experiment was repeated independently three times with similar results.
