Extended Data Fig. 5: Kinetics of endogenous HNO and •NO formation under different cellular redox states in WT Arabidopsis leaves.
From: Discovery of endogenous nitroxyl as a new redox player in Arabidopsis thaliana
a, The effect of a menadione-induced (0.1 mM MN) redox environment on HNO/•NO generation. b, The effect of an N-acetylcysteine-induced (1 mM NAC) redox environment on HNO/•NO generation. c, Neither redox modulator significantly changed cell viability of Arabidopsis WT leaves. Leaves treatment with 0.1 mM MN shifted the cellular environment toward oxidative, manifested by a reduced GSH/GSSH ratio, an elevated level of hydrogen peroxide, and superoxide (d-f). Leaves treatment with 1 mM NAC shifted the cellular environment toward reductive by enhanced GSH/GSSH ratio, the elevated pool of reduced glutathione, and an improved total antioxidant capacity (f-h). Data are presented as mean ± s.d. of three biologically independent replicates (n = 3). (*) indicate values that differ significantly (P ≤ 0.05) from control (untreated) WT leaves. Statistical significance was assessed using two-tailed t-tests.
