Extended Data Fig. 6: Nitroxyl implications in dark-induced leaf senescence (DILS) of WT Arabidopsis.
From: Discovery of endogenous nitroxyl as a new redox player in Arabidopsis thaliana
a, Redox parameters of leaf cells undergoing DILS. H2O2, hydrogen peroxide; TEAC, total antioxidant activity; GSH/GSSH ratio, the ratio of reduced to oxidized glutathione. Black arrows indicate a three-day recovery phase by reversing the DILS program by restoring light access on day 3. b, The effect of applying exogenous HNO as 1.5 mM CM on the expression of senescence-associated genes (SAGs), SAG12, SAG20, and SEN4, which are molecular markers of the changes underpinning senescence62. c, Chlorophyll content in leaves undergoing DILS. Data are presented as mean ± s.d. of three biologically independent replicates (n = 3). (*) indicates values that differ significantly (P ≤ 0.05) from (a) control leaves or day 3 of DILS in case of recovery experiment, or (b, c) untreated leaves undergoing DILS. Statistical significance was assessed using two-tailed t-tests.
