Fig. 1: eINTACT enables the isolation of nuclei from effector-recipient host cells.
From: The eINTACT system dissects bacterial exploitation of plant osmosignalling to enhance virulence
a, Schematic of the Arabidopsis-Xcc eINTACT system (Supplementary Text). b, Isolation of the biotin-tagged nuclei using streptavidin-coated magnetic beads by affinity-based purification (INTACT method, Supplementary Text). c, Reverse transcription-polymerase chain reaction (RT-PCR) detection of NTF expression in Xcc*vec1 and Xcc*AvrBs3 infected leaves, at seven days postinfection (DPI). The expression of TUB2 is used as a control. d–f, Microscopy images of leaf cells that have received Xcc*AvrBs3 effectors. Scale bar, 50 μm. g–i Microscopy images of eINTACT-purified nuclei (indicated with white triangles) from Xcc*AvrBs3 infected leaves. Scale bar, 100 μm. In microscopy images, BF indicates bright field (d,g); magnetic beads appear as white spheres (g); DNA stained with DAPI is shown in blue (e,h); and nuclear envelope domains tagged with red NTF are detected by mCherry fluorescence (f,i). Three repeats of each experiment (c–i) were performed independently with similar results.
