Fig. 1: Dynamics of accessible chromatin landscape during stomatal cell-state progression.

a, Schematic of stomatal development (top) with representative confocal images (bottom) of stomatal lineage cell-type-specific markers of each INTACT line (RFP) in LTi6b (GFP plasma membrane marker) background. These INTACT lines are used for monitoring the genome-wide chromatin accessibility by ATAC-seq. Each colour bar represents the duration of expression of each gene: AtML1 (grey), SPCH (purple), MUTE (cyan), FAMA (light green), GC (green). *Note that AtML1 is expressed predominantly in the protodermal cells, and its modest expression persists in the stomatal-lineage cells. Scale bar, 10 μm. The experiment was repeated independently three times with similar results. b, Genome browser view of differential chromatin accessibility sites showing the specificity of INTACT nuclei preparations. c, Genome browser view of static (SCRM/ICE1) and dynamic (ERECTA) chromatin accessibility sites of the endogenous locus encoding known regulators of stomatal development. d, Heat map showing differentially accessible sites during cell-state transition in stomatal development. For each THS, accessibility values are shown relative to the maximum value across stomatal developmental stage.e, A line plot trajectory of normalized chromatin accessibility shows two distinct clusters during stomatal development. ELC, early lineage specific cluster (cyan); PCC, post commitment cluster (green).