Extended Data Fig. 9: Relative expression analysis for early stomatal precursor marker genes in WT, bpc mutants, and BPC inducible overexpression lines.

(a) Loss of BPC1 and BPC2 induce the expression early stomatal precursor marker genes, EPF2, BASL, POLAR, and TMM. Conversely, they are repressed by induced overexpression of iBPC1 (b) and iBPC2 (c). Relative expression represents qRT-PCR values normalized over that of ACTIN2. Student’s t-test was performed for pairwise comparisons: *, p < 0.05; **, p < 0.005; ***, p < 0.0005; ****, p < 0.00005; ns, not significant. Error bars, s.e.m. (n = 3) Three biological replicates were performed, each with 3 technical replicates. See Supplementary Table 9 for all exact p values.