Extended Data Fig. 9: Positions of the Rieske head domain in complex III structures. | Nature Plants

Extended Data Fig. 9: Positions of the Rieske head domain in complex III structures.

From: Cryo-EM structure of the respiratory I + III2 supercomplex from Arabidopsis thaliana at 2 Å resolution

Extended Data Fig. 9

In the Arabidopis I + III2 supercomplex (a), the Rieske head domain (magenta) is locked in the b-state with its FeS cluster positioned 6.8 Å and H237 2.9 Å away from the bound ubiquinone/ubiquinol that accepts the electron. These distances resemble those in the stigmatellin-bound complex III2 crystal structure of Saccharomyces cerevisiae (b) and are smaller than in the b-state in the cryo-EM structures of the Ovis aries (sheep) I + III2 supercomplex (c) and of the Candida albicans complex III2 (d) where the Rieske head group was also resolved in the c-state (e). Ubiquinone/ubiquinol is shown in pink, the inhibitor Stigmatellin in blue; distances between Qo/Rieske FeS group and Qo/FeS coordinating histidine are indicated by black dotted lines. PDB accession codes: Saccharomyces, 1EZV43; Ovis, 6Q9E25; Candida b-state, 7RJB, and Candida c-state, 7RJD81. Sidechains of the Rieske head domain histidine are not present in the Candida models.

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