Extended Data Fig. 2: Detection of paternal plastid transmission in the absence of selection.
From: Control of plastid inheritance by environmental and genetic factors
a, Seeds harvested from crossing experiments were germinated in medium without antibiotics. Scale bar, 10 mm. b, Stereomicroscopic images of a seedling containing paternally inherited plastids grown in antibiotic-free medium. The microscopic bright field picture (left), the GFP fluorescence image (middle) and the chlorophyll fluorescence image (right) are shown. Arrowheads 1 and 2 point to two sectors containing paternal plastid as evidenced by GFP fluorescence. Scale bar, 1 mm. c, Visualization of paternal plastids by their antibiotic resistance. The seedling shown in b was transferred to spectinomycin-containing medium that causes bleaching of cells that contain only maternal plastids. Photographs were taken 7 days (left; scale bar, 1 mm), 20 days (middle; scale bar, 1 mm) and 48 days (right; scale bar, 10 mm) after transfer. d, Frequency of paternal plastid transmission observed in the absence of spectinomycin selection.
