Table 1 Crossing experiments and screens for paternal plastid transmission by spectinomycin selection
From: Control of plastid inheritance by environmental and genetic factors
Treatment | Abiotic stress applied | Paternal genetic background | Harvest | Seeds screened | No. of paternal transmission events | Paternal plastid transmission rate (%) | |
|---|---|---|---|---|---|---|---|
Exp. 1 | Standard | − | WTptGFP | 1 | 942,992 | 16 | 0.0017 |
2 | 238,423 | 6 | 0.0025 | ||||
3 | 637,379 | 10 | 0.0016 | ||||
4 | 275,794 | 1 | 0.0004 | ||||
Total | 2,094,588 | 33 | 0.0016 | ||||
High Light | 1,000 μE m−2 s−1 | WTptGFP | 1 | 152,719 | 2 | 0.0013 | |
2 | 146,631 | 1 | 0.0007 | ||||
3 | 174,965 | 0 | − | ||||
Total | 474,315 | 3 | 0.0006 | ||||
Heat | 35 °C | WTptGFP | 1 | 247,451 | 0 | − | |
2 | 248,615 | 1 | 0.0004 | ||||
3 | 231,540 | 3 | 0.0013 | ||||
Total | 727,606 | 4 | 0.0005 | ||||
Drought | No watering until visible wilting occurred | WTptGFP | 1 | 173,077 | 2 | 0.0012 | |
2 | 131,329 | 4 | 0.0030 | ||||
3 | 167,625 | 4 | 0.0024 | ||||
Total | 472,031 | 10 | 0.0021 | ||||
Chilling | 10 °C | WTptGFP | 1 | 167,169 | 286 | 0.1711 | |
2 | 147,661 | 402 | 0.2722 | ||||
3 | 164,400 | 463 | 0.2816 | ||||
Total | 479,230 | 1,151 | 0.2402 | ||||
Exp. 2 | Standard | − | WTptGFP | 14,060 | 0 | − | |
dpd1ptGFP | 18,060 | 24 | 0.1329 | ||||
Chilling | 10 °C | WTptGFP | 9,560 | 16 | 0.1674 | ||
dpd1ptGFP | 9,060 | 198 | 2.1854 | ||||
Exp. 3 | Chilling | 10 °C | WTptGFP | 12,000 | 31 | 0.2583 | |
dpd1ptGFP | 12,500 | 396 | 3.1680 |
