Table 1 Interactors of NCR169 promoter regions identified in Y1H library screens (1,181 bp) and DNA affinity chromatography (382 bp) from both M. truncatula and G. max

From: Widely conserved AHL transcription factors are essential for NCR gene expression and nodule development in Medicago

Interacting proteins

Gene ID

UniProt ID

Proteins interacting with 1,181bp NCR169 promoter in Y1H screening

 MtAHL-1

Medtr4g098450

G7JGG2

 Basic helix–loop–helix domain class transcription factor

Medtr4g081370

B7FHK4

 MYB-like transcription factor

Medtr5g037080

G7K6Z9

 TCP family transcription factor

Medtr7g028160

G7L283

 Transcription factor VOZ1-like protein

Medtr4g088125

A0A072UMQ9

 BEL1-related homeotic protein

Medtr8g098815

A0A072TUA6

 Linker histone H1 and H5 family protein

Medtr6g079520

A0A072UBH0

M. truncatula proteins interacting with 382bp NCR169 promoter in DNA pull-down assay

 MtAHL1

Medtr4g098450

G7JGG2

 MtAHL2

Medtr7g080980

G7KSI4

 MtPHD1

Medtr4g015830

A0A072UGM4

 MtPHD2

Medtr1g015185

A0A072VDQ0

 Myb protein

Medtr7g020870

G7KSV7

 Myb protein

Medtr7g103390

A0A072U4N3

 MtPurα

Medtr4g083230

G7JHJ7

 Zinc finger protein

Medtr1g053960

A0A072VIQ3

G. max proteins interacting with 382bp NCR169 promoter in DNA pull-down assay

 GmAHL1

Glyma.18G247200

C6TMY6

 GmPHD1

Glyma.09G107000

C6T7X8

 GmPurα

Glyma.07G225200

I1KMC5

 SHOOT2 protein

Glyma.03G189600

C6TI90

 Trihelix protein

Glyma.18G281800

I1N4Y2