Extended Data Fig. 7: HEM1 condensation behaviors in plants during immune activation.
From: Plant HEM1 specifies a condensation domain to control immune gene translation
a, ETI induction in N. benthamiana by recognition between β-estradiol-controlled bacterial effector AvrPto and the corresponding constitutively-expressed host target Pto. mYFP-HEM1 condensation by ETI activation through treatment with β-estradiol (+) with water as a control (–). Scale bars, 10 µm. b, mYFP-HEM1 condensation in N. benthamiana by SA at indicated concentrations and durations. Scale bars, 10 µm. c, Condensation behaviors of different mYFP-tagged HEM1 regions. Data are shown as mean ± s.d. (n = 10) of condensate numbers in each construct. One-way ANOVA with post-hoc analysis by Tukey test. d, HEM1ΔLCD and LCD alone decrease the condensation capacity by SA, and LCD alone has mislocalization to an unknown ring structure (arrowhead). Assays were performed in N. benthamiana. Scale bars, 10 µm. e, f, Detecting HEM1 condensates in the pellet fraction after SA treatment by microscopy (e) and immunoblot analysis of HEM1 (f). Scale bars, 5 µm. Experiments have been repeated three times with similar results. g, h, GO term of HEM1 interactome identified in the soluble (g) and pellet (h) fractions after SA treatment using the online ShinyGO v0.741. P value (g, h), Hyper Geometric test. g, Venn diagram of the HEM1 interactome in the soluble and pellet fractions. Translation factors are shown.
