Extended Data Fig. 8: HEM1 condensation with translation factors during immune activation by SA treatment.
From: Plant HEM1 specifies a condensation domain to control immune gene translation
a, Interaction of HEM1 with nCBP and eIF3d-2 by BiFC. Transient expression of nYFP-HEM1 with nCBP-cYFP or eIF3d-2-cYFP was done in N.benthamiana. Scale bars, 10 µm. b, Co-localization assay to show LCD-dependence for co-existence in the condensate. Transient expression of mYFP-HEM1 with nCBP-CFP or eIF3d-2-CFP was done in N.benthamiana. Arrowheads indicate condensates. Scale bars, 10 µm. c, nCBP condensation in Arabidopsis by SA treatment depends on HEM1. 35 S:YFP-nCBP transgenic plants were generated and crossed into hem1. Scale bars, 10 µm. d, Immunoblot analysis of YFP-nCBP in the soluble and pellet fractions of Col-0 and hem1 after SA treatment. Experiments have been repeated three times with similar results.
