Extended Data Fig. 2: HEM1 in immune responses.
From: Plant HEM1 specifies a condensation domain to control immune gene translation
a, MAPK activation. Three-week-old plants were infiltrated with 1 μM elf18 solution and collected at the indicated time points for immunoblot analysis using the phosphospecific antibody against MAPK3 and MAPK6. CBB, coomassie brilliant blue staining. b, Callose deposition. Three-week-old plants were infiltrated with 1 μM elf18 or Mock. Scale bars 100 μm. c, The elf18-induced resistance to Psm ES4326. Mean ± s.d. (n = 8). d, e, mYFP-HEM1 under its native promoter complements the LUC (d, upper; scale bars, 1 cm), growth (d, middle; scale bars, 1 cm) and trichome (d; bottom; scale bars, 1 mm), and ETI cell death (e; scale bars, 1 cm) phenotypes of hem1. efr-1, defective in the elf18 recognition receptor EFR. Line1/2, two independent genetic complementation lines with mYFP-HEM1 under its native promoter in the hem1 background.
