Extended Data Fig. 6: PAT in root tips.
From: Gibberellins promote polar auxin transport to regulate stem cell fate decisions in cambium
a, A schematic explaining the setup of the PAT assay. The red circle indicates the position of 3H-IAA application, black arrow showing the direction of IAA movement. The black line marks the area sampled to detect 3H-IAA. b, 3H-IAA transport from the root-shoot transition zone to the root tips after a 1 h GA treatment in 6-day old seedlings of Col-0 and various mutants. After 0, 1, or 2 days, the plants were treated with 3H-IAA for 3 h and then sampled. Data shown are means ± SD (n = 3 independent pools of 10). Individual data points are plotted as purple dots. c–d, Side-view of DR5rev:GUS expression after 2-day GA4,7 or mock treatment on 8-day old plants in Col-0 (c) and pin1 (d) backgrounds. Numbers on the lower right corner are quantifications on how many plants look like the shown example. Scale bar are 50 µm (c,d). Two-way ANOVA with Tukey’s post hoc test in b. Bars not sharing a lower-case letter in the plots indicate a significant difference, P < 0.05.
