Extended Data Fig. 9: Transcriptomic characterization of flower buds in comparison of the original male and the sygl (edited) lines.

a, Principal component analysis (PCA) of gene expression profiles in inflorescences of male and sygl hermaphrodite lines. Day 0 and day 4 represented embryonic inflorescence from dormant and breaking buds, respectively. Day 10 and day 14 represented inflorescences developing in leaf axils of expanding shoots. b, Representative images demonstrating early inflorescence development. Comparable development was observed in male and sygl lines with dome shaped meristems (dsm) and early inflorescence (inf) and bract (br) development at day 0, initiation of lateral flowers (lf) and sepals (s) at day 4, and presence of lateral and terminal flowers (tf) at day 10, but only terminal and aborted lateral flowers (alf) were present in sygl lines at day 14. c, Heatmap and hierarchical clustering of the DEGs between the male and the sygl-edited hermaphrodite inflorescences. Clust 2 (d) and Clust 5 (f) include genes upregulated in the sygl-edited hermaphrodite during the time period of day 4 to 10, and constantly downregulated in the sygl-edited hermaphrodite for the time period from day 0 to 14, respectively (Supplementary Data S5). GO analysis of Clust2 (e), and Clust5 (g).