Extended Data Fig. 1: Activity of mammalian and plant codon-optimized TbID in Arabidopsis protoplasts.
From: TurboID-based proteomic profiling of meiotic chromosome axes in Arabidopsis thaliana
(a) Immunoblots (IB) of protein extracts from protoplasts transformed with mammalian or plant codon-optimized TbID and treated with 0.05 mM biotin for 2 hrs. Anti-HA antibody and Streptavidin (SA) used for blotting. Protein extract from non-transformed protoplasts used as control. The experiment was repeated three times independently with similar results. (b) DNA sequence of custom synthesized TbID (plant codon-optimized).
