Extended Data Fig. 8: Identification of CRISPR/Cas9–edited GhDIR5 knockout lines.
From: Dirigent gene editing of gossypol enantiomers for toxicity-depleted cotton seeds
a, Western blot detection of leaf proteins after SDS-PAGE. GhDIR5 antibody (Abmart) was used at a dilution of 1:1000, using goat-anti-mouse IgG horseradish peroxidase conjugate (1:10000, ZB2305, ORIGENE) as secondary antibody. The experiments were repeated independently three times with similar results. b-c, CRISPR/Cas9-introduced GhDIR5 mutation didn’t led to off-target mutations at the GhDIR4 (b) and GhDIR6 (c) loci, as determined by Sanger sequencing after PCR amplification.
