Extended Data Fig. 2: Phylogenetic analysis of the DIR family proteins from upland cotton (n = 121), rice (n = 49), and Arabidopsis thaliana (n = 26).
From: Dirigent gene editing of gossypol enantiomers for toxicity-depleted cotton seeds
Proteins whose function has been described in the literature are indicated. [AtDIR6 and AtDIR5, (−)-pinoresinol-forming DIRs from A. thaliana69, in the DIR-a subfamily; AtDP1/AtDIR12, a neolignan biosynthesis DIR from A. thaliana52, in the DIR-a subfamily; AtDIR10 (ESB1), a Casparian band lignin-forming DIR from A. thaliana53, in the DIR-e subfamily; GhDIR1, a DIR involved in cotton lignification which could limit the spread of fungal pathogen Verticillium dahlia70, in the DIR-b/d subfamily]. GhDIR5, GhDIR6 and the previously reported GhDIR412 and GhDIR371 (another Gossypium hirsutum DIR for (+)-2 formation) are classified to the DIR-b/d subfamily, in which GhDIR6 belongs to a branch separated earlier.
