Extended Data Fig. 4: Subcellular localization of GhDIR5 and GhDIR6.
From: Dirigent gene editing of gossypol enantiomers for toxicity-depleted cotton seeds
a, Confocal microscopy (Leica TSC SP8 STED 3X) of pGhDIR5::GhDIR5-EGFP and pGhDIR6::GhDIR6-EGFP transgenic cotton leaves. Scale bars, 30 μm. b, GUS staining of pGhDIR5::GUS and pGhDIR6::GUS transgenic cotton leaves. Scale bars, 50 μm. c, Immunoelectron microscopy with monoclonal antibodies against GhDIR5 and gold-labelled secondary antibodies, showing the localization of GhDIR5 in pigmented glands. The magnification of the box is shown in the panel below. Arrows indicate the immunogold particles in cotton pigmented glands. Scale bars of original and amplified panels are 10 μm and 500 nm, respectively. For controls the primary antibodies were replaced with the non-immune serum. All experiments were repeated independently at least two times with similar results, representative images are shown.
