Extended Data Fig. 2: SIG5 transcript accumulation during cold treatments of up to 24 h.
From: Low-temperature and circadian signals are integrated by the sigma factor SIG5
SIG5 transcript accumulation during cold treatments of up to 24 h. (A) Relative abundance of SIG5 transcripts in Col-0 wild type and sig5-3 plants after cold treatment (4 °C) for durations ranging from 30 mins to 24 hours. (B) Abundance of SIG5 transcripts in wild type (Col-0) and two athb17 mutants after 3 h at 19 °C or 4 °C. Plants were under constant light conditions and given a 3 h cold treatment 1 h after subjective dawn. Experiments used 11-day old seedlings. Data are mean + /- s.e.m. (n = 3 independent biological replicates). Statistical comparisons are of (A) transcript abundance between Col-0 19 °C and Col-0 4 °C and (B) transcript abundance between control temperature and cold-treated plants, and between genotypes. Data analysed by (A) one-way ANOVA and (B) two-way ANOVA, both followed by one-sided post-hoc Tukey test, where *** = p < 0.001; ** = p < 0.01, * = p < 0.05 and n.s = not significant. p-values (A) 19 °C vs 4 °C for Col-0 at 0 h (p = 1.0000), 0.5 h (p = 0.9277), 1 h (p = 1.0000), 3 h (p = 0.1867), 6 h (p < 0.0001), 9 h (p < 0.0001), 12 h (p = 0.0001), 24 h (p < 0.0001); (B) 19 °C vs 4 °C for Col-0 (p = 0.0000425), athb17-1 (p = 0.0008523); athb17-2 (p = 0.0010056); Col-0 vs athb17-1 at 4 °C (p = 0.1958629); Col-0 vs athb17-2 at 4 °C (p = 0.0918899).
