Extended Data Fig. 5: The identification of cenh3-8 allele. | Nature Plants

Extended Data Fig. 5: The identification of cenh3-8 allele.

From: A simple and highly efficient strategy to induce both paternal and maternal haploids through temperature manipulation

Extended Data Fig. 5

a, The cenh3-8 resulted from genome editing by CRISPR has a 276 base pair (bp) deletion in CENH3 locus. The gene structure of CENH3 is shown at top panel; the protospacer adjacent motif (red font) and 20-base pair target sequence of the gRNA (highlighted in yellow) are shown; the cenh3-8 is a 276 base pair deletion (highlighted in red) mutant. b, Protein alignment for CENH3-WT, CENH3-8 by using ClustalW. The protein of CENH3-8 has a 27 amino acids deletion at the N-terminal tail. c, A representative photograph for Col-0, GFP-tailswap, and cenh3-8 at the rosette stage. Scale bar, 1 cm. d, The viability of pollen grains of Col-0 and cenh3-8 was tested by coloration with Alexander’s stain, the Col-0 and cenh3-8 plants were grown at 18 °C, 22 °C, and 25 °C. Twice experiments were repeated independently with similar results. Scale bar, 100 μm.

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