Extended Data Table 1 Identification of GFP-tailswap haploid induction efficiency under low and high temperature conditions

From: A simple and highly efficient strategy to induce both paternal and maternal haploids through temperature manipulation

  1. Seeds from five siliques collected from outcrossing of GFP-tailswap (18 °C and 25 °C) by pollen of Ler (22 °C); On the basis of shrink level, seeds are divided into four groups: dark inviable seeds, wrinkled seeds (size < 1/2), wrinkled seeds (size > 1/2), and plump viable seeds; then transfer each group of seeds into 1/2 MS medium and grow. After two weeks, gemination rates were counted, and the resulting seedlings were transplanted into nutrient soil, and count the proportions of haploids, aneuploids, and diploids. Percentage values in bracket are the proportions of haploid, aneuploid and diploid in all surviving plants. The HIR of GFP-tailswap tested at 18 °C and 25 °C using Percival (AR-41L3) plant growth chamber.
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