Extended Data Fig. 1: slrx620 mutation suppresses the phenotypes of lrx345 mutant.
From: FERONIA coordinates plant growth and salt tolerance via the phosphorylation of phyB
a, Phenotype of wild type, lrx345, and slrx620 mutant grown on 1/2 MS medium with or without NaCl (120 mM) under white light conditions (16 h light/8 h dark cycle). b, Quantification of the survival rate of seedlings grown on NaCl medium. Values are the means ± SD of three biological replicates. c, Representative images of wild type, lrx345, and slrx620 mutant grown on soils for 22 days under LD conditions. Scale bar, 1 cm. d, Quantification of the petiole length of plants shown in c. Values are the means ± SD (n = 11 seedlings). e, RT–qPCR analysis of the transcript levels of PDF1.2 and PDF1.3 genes in each genotype. Values are the means ± SD of three biological replicates. f, Identification of mutations in slrx620 mutant by bulked segregant analysis (BSA). Red asterisk indicates the location of the mutated gene that is associated with the slrx620 mutant phenotypes. g, Diagram illustrating the genomic structure of phyB and the mutation site in the slrx620 mutant. h. Hypocotyl growth of seedlings grown under white light conditions for 4 days. Scale bar, 2 mm. i, Quantification of the hypocotyl length of the seedlings shown in h. Values are the means ± SD (n = 49-63 seedlings). Asterisks in b, d and e indicate statistically significant differences (**P < 0.01, Student’s t test, two-sided). Different letters in i indicate statistically significant differences (P < 0.01, one-way ANOVA).
