Extended Data Fig. 5: Sequence alignment of Lhca2 isoforms and the assignment of the protein subunit in the structure of Pp PSI-LHCI-LHCII-Lhcb9.
From: Structural insights into a unique PSI–LHCI–LHCII–Lhcb9 supercomplex from moss Physcomitrium patens
a, Sequence alignment of five Lhca2 isoforms of P. patens. b, Assignment of Lhca2.2 by comparison of the map features of characteristic residues and the corresponding sequences. Lhca2.2 contains residues Phe69, Ala148, Pro150, Asn174, Val237, Leu252, Gly259 and Phe264 which fit well with the density map, which excludes the existence of all other Lhca2 isoform proteins. c, Assignment of Lhca2.5 by comparison of the map features of characteristic residues and the corresponding sequence. Lhca2.5 contains residues Leu69, Asn101, Tyr139, Lys140, Gln150, Arg174, Phe237, Phe252, Tyr259and Leu264 which fit well with the density map, which excludes the existence of all other Lhca2 isoform proteins. The red triangles show characteristic residues used for identification. Sequences are taken from Uniprot as follows: Lhca2.1[PHYPADRAFT_178694 - Chlorophyll a-b binding protein, chloroplastic - Physcomitrium patens (Spreading-leaved earth moss) - PHYPADRAFT_178694 gene & protein (uniprot.org)]. Lhca2.2[PHYPADRAFT_151155 - Chlorophyll a-b binding protein, chloroplastic - Physcomitrium patens (Spreading-leaved earth moss) - PHYPADRAFT_151155 gene & protein (uniprot.org)]. Lhca2.3[PHYPA_025494 - Chlorophyll a-b binding protein, chloroplastic - Physcomitrium patens (Spreading-leaved earth moss) - PHYPA_025494 gene & protein (uniprot.org)]. Lhca2.4[PHYPADRAFT_63624 - Chlorophyll a-b binding protein, chloroplastic - Physcomitrium patens (Spreading-leaved earth moss) - PHYPADRAFT_63624 gene & protein (uniprot.org)]. Lhca2.5[PHYPA_014361 - Chlorophyll a-b binding protein, chloroplastic - Physcomitrium patens (Spreading-leaved earth moss) - PHYPA_014361 gene & protein (uniprot.org)].
