Extended Data Fig. 7: Semi-quantitative analysis of coimmunoprecipitation assay.
From: NSF/αSNAP2-mediated cis-SNARE complex disassembly precedes vesicle fusion in Arabidopsis cytokinesis
(a-b) Group box plots of semi-quantitative analysis. Signal intensities of the immunoprecipitate (IP) fractions with anti-GFP (a) and anti-RFP (b) beads were measured from the two or three immunoblots that were probed with Q-SNARE antisera as in Fig. 1i,j: SYP132, SYP121, SYP61, SYP31, SYP21, SYP81. Band intensity values were normalized with NSF:G and R:αS2 set at 100; A.U, arbitrary units. For simplicity, IP fractions from the non-transformed wild type extract were not shown. The P values were calculated using unpaired Student’s t-test (two-tailed) by comparing the respective wild type to its dominant negative mutant. The center line of the box plots is the median, the bottom and top lines represent the lower quartile and upper quartile, respectively. n, number of the counted immunoblots. Note that P value was not calculated (n.c) for n≤2.
