Extended Data Fig. 10: Interference with KEULE localization at the cell division plane by αSNAP2 dominant negative variant.
From: NSF/αSNAP2-mediated cis-SNARE complex disassembly precedes vesicle fusion in Arabidopsis cytokinesis
(a-h) KNOLLE (KN) and KEULE localization in dividing cells of seedling roots after estradiol induction of wild-type R:αS2 (red, a-d) or dominant-negative αS2LA (red, e-h). (b, f) anti-KN antiserum (blue); (c, g) vYFP (vY):KEULE (green); same images as shown in Fig. 3d–i; (a, e) RFP channels; (d, h) merged images; scale bars, 5 µm (d, h). Double arrowheads, cell division plane region (a, e). (i) Localization efficiency of KEULE at the cell division plane (CDP). The P value was calculated using two-tailed Welch’s t-test by comparing R:αS2 to R:αS2LA. The center line of the box plots is the median, the bottom and top lines represent the lower quartile and upper quartile, respectively. Data points are shown as jitter. n, number of images comprising 40 cytokinetic cells in R:αS2 and 119 cells in R:αS2LA that were analysed in total, respectively. Numbers of seedlings analysed: 24 (a-d); 40 (e-h).
