Extended Data Fig. 2: Schematic of NSF and αSNAP2 and impact of their dominant-negative variants on Arabidopsis embryo development.
From: NSF/αSNAP2-mediated cis-SNARE complex disassembly precedes vesicle fusion in Arabidopsis cytokinesis
(a) Schematic of NSF and αSNAP2. NSF domains indicated; N domain, interaction with αSNAP2. Numbers, amino acid positions. Residues altered in dominant-negative variants highlighted in red: E326 (NSF), L288 (αSNAP2). (b-m) Embryo abnormalities caused by RPS5A (R)»UAS two-component expression of NSFEQ:G (GFP-tagged dominant-negative NSFE326Q, b-g) or R:αS2LA (RFP-tagged dominant-negative αSNAP2L188A, h-m). Non-transgenic wild-type control embryo (n-p); (b, e, h, k, n) cell-wall staining with Renaissance blue 2200; (c, f, i, l, o) FP, fluorescent fusion proteins (c and f, GFP; i and l, RFP; o, no FP fluorescence); (d, g, j, m, p) bright-field Images; scale bars, 10 µm. Note that the mutants were clearly discernible around the heart stage, developing severe abnormalities thereafter (e-g, k-m). Number of embryos analysed, ≥300.
