Extended Data Fig. 5: Effect of dominant-negative NSF on endocytosis.
From: NSF/αSNAP2-mediated cis-SNARE complex disassembly precedes vesicle fusion in Arabidopsis cytokinesis
(a-l) Live imaging of NSF:G (a-c) or NSFEQ:G (d-f) and endocytic tracer SynaptoRedTM C2 in seedling roots after 24 hours of EST induction. (g-l) Imaging of NSF:G (g-i) or NSFEQ:G (j-l) and SynaptoRedTM C2 upon brefeldin A (BFA) application. (m) Cytosolic intensity quantification of SynaptoRedTM C2 signals. Cytosolic area in a single cell was selected for quantification. The P value was calculated using two-tailed Welch’s t-test by comparing NSF:G to NSFEQ:G. The center line of the box plots is the median, the bottom and top lines represent the lower quartile and upper quartile, respectively. Data points are shown as jitter. n, number of counted cells.
